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Structure paper

TitleA Comprehensive Structure-Function Comparison of Hepatitis C Virus Strains Jfh1 and J6 Polymerases Reveals a Key Residue Stimulating Replication in Cell Culture Across Genotypes.
Journal, issue, pagesJ. Virol., Vol. 85, Page 2565-, Year 2011
Publish dateNov 3, 2010 (structure data deposition date)
AuthorsSchmitt, M. / Scrima, N. / Radujkovic, D. / Caillet-Saguy, C. / Simister, P.C. / Friebe, P. / Wicht, O. / Klein, R. / Bartenschlager, R. / Lohmann, V. / Bressanelli, S.
External linksJ. Virol. / PubMed:21209117
MethodsX-ray diffraction
Resolution1.774 - 1.881 Å
Structure data

PDB-2xwh:
HCV-J6 NS5B polymerase structure at 1.8 Angstrom
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-2xxd:
HCV-JFH1 NS5B polymerase structure at 1.9 angstrom
Method: X-RAY DIFFRACTION / Resolution: 1.881 Å

PDB-2xym:
HCV-JFH1 NS5B T385A mutant
Method: X-RAY DIFFRACTION / Resolution: 1.774 Å

Chemicals

ChemComp-15P:
POLYETHYLENE GLYCOL (N=34) / precipitant*YM

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-HOH:
WATER

ChemComp-PO4:
PHOSPHATE ION

Source
  • hepatitis c virus isolate hc-j6
  • hepatitis c virus
KeywordsTRANSFERASE / REPLICATION / HEPACIVIRUS / NONSTRUCTURAL PROTEINS / DE NOVO INITIATION / PRIMING

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