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TitleEngineering encodable lanthanide-binding tags into loop regions of proteins.
Journal, issue, pagesJ. Am. Chem. Soc., Vol. 133, Page 808-819, Year 2011
Publish dateFeb 16, 2010 (structure data deposition date)
AuthorsBarthelmes, K. / Reynolds, A.M. / Peisach, E. / Jonker, H.R. / DeNunzio, N.J. / Allen, K.N. / Imperiali, B. / Schwalbe, H.
External linksJ. Am. Chem. Soc. / PubMed:21182275
MethodsX-ray diffraction
Resolution1.7 - 2.1 Å
Structure data

PDB-3ltq:
Structure of Interleukin 1B solved by SAD using an inserted Lanthanide Binding Tag
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-3pok:
Interleukin-1-beta LBT L3 Mutant
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

Chemicals

ChemComp-TB:
TERBIUM(III) ION

ChemComp-ACT:
ACETATE ION

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

Source
  • homo sapiens (human)
KeywordsCYTOKINE / Lanthanide Binding Tag / LBT / terbium / IL1B / Inflammatory response / Mitogen / Pyrogen / Secreted / lanthanide tag / beta trefoil / interleukin / signaling / receptor / extracellular membrane

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