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-Structure paper
Title | RNA channelling by the eukaryotic exosome. |
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Journal, issue, pages | EMBO Rep, Vol. 11, Issue 12, Page 936-942, Year 2010 |
Publish date | Nov 12, 2010 |
Authors | Hélène Malet / Maya Topf / Daniel K Clare / Judith Ebert / Fabien Bonneau / Jerome Basquin / Karolina Drazkowska / Rafal Tomecki / Andrzej Dziembowski / Elena Conti / Helen R Saibil / Esben Lorentzen / |
PubMed Abstract | The eukaryotic exosome is a key nuclease for the degradation, processing and quality control of a wide variety of RNAs. Here, we report electron microscopic reconstructions and pseudo-atomic models ...The eukaryotic exosome is a key nuclease for the degradation, processing and quality control of a wide variety of RNAs. Here, we report electron microscopic reconstructions and pseudo-atomic models of the ten-subunit Saccharomyces cerevisiae exosome in the unbound and RNA-bound states. In the RNA-bound structures, extra density that is visible at the entry and exit sites of the exosome channel indicates that a substrate-threading mechanism is used by the eukaryotic exosome. This channelling mechanism seems to be conserved in exosome-like complexes from all domains of life, and might have been present in the most recent common ancestor. |
External links | EMBO Rep / PubMed:21072061 / PubMed Central |
Methods | EM (single particle) |
Resolution | 12.0 - 14.0 Å |
Structure data | EMDB-1708: EMDB-1709: |
Source |
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