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Structure paper

TitleEvolution of I-SceI Homing Endonucleases with Increased DNA Recognition Site Specificity.
Journal, issue, pagesJ. Mol. Biol., Vol. 405, Page 185-200, Year 2011
Publish dateAug 31, 2010 (structure data deposition date)
AuthorsJoshi, R. / Ho, K.K. / Tenney, K. / Chen, J.H. / Golden, B.L. / Gimble, F.S.
External linksJ. Mol. Biol. / PubMed:21029741
MethodsX-ray diffraction
Resolution2.3 - 2.5 Å
Structure data

PDB-3ool:
I-SceI complexed with C/G+4 DNA substrate
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-3oor:
I-SceI mutant (K86R/G100T)complexed with C/G+4 DNA substrate
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-HOH:
WATER

Source
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsHYDROLASE/DNA / homing endonuclease / intron homing / LAGLIDADG / Hydrolase-DNA complex / I-SceI mutant (K86R/G100T)

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