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TitleInsights into egg coat assembly and egg-sperm interaction from the X-ray structure of full-length ZP3.
Journal, issue, pagesCell, Vol. 143, Issue 3, Page 404-415, Year 2010
Publish dateOct 29, 2010
AuthorsLing Han / Magnus Monné / Hiroki Okumura / Thomas Schwend / Amy L Cherry / David Flot / Tsukasa Matsuda / Luca Jovine /
PubMed AbstractZP3, a major component of the zona pellucida (ZP) matrix coating mammalian eggs, is essential for fertilization by acting as sperm receptor. By retaining a propeptide that contains a polymerization- ...ZP3, a major component of the zona pellucida (ZP) matrix coating mammalian eggs, is essential for fertilization by acting as sperm receptor. By retaining a propeptide that contains a polymerization-blocking external hydrophobic patch (EHP), we determined the crystal structure of an avian homolog of ZP3 at 2.0 Å resolution. The structure unveils the fold of a complete ZP domain module in a homodimeric arrangement required for secretion and reveals how EHP prevents premature incorporation of ZP3 into the ZP. This suggests mechanisms underlying polymerization and how local structural differences, reflected by alternative disulfide patterns, control the specificity of ZP subunit interaction. Close relative positioning of a conserved O-glycan important for sperm binding and the hypervariable, positively selected C-terminal region of ZP3 suggests a concerted role in the regulation of species-restricted gamete recognition. Alternative conformations of the area around the O-glycan indicate how sperm binding could trigger downstream events via intramolecular signaling.
External linksCell / PubMed:20970175
MethodsX-ray diffraction
Resolution2 - 2.6 Å
Structure data

PDB-3nk3:
Crystal structure of full-length sperm receptor ZP3 at 2.6 A resolution
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

PDB-3nk4:
Crystal structure of full-length sperm receptor ZP3 at 2.0 A resolution
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-FLC:
CITRATE ANION

ChemComp-HOH:
WATER

ChemComp-A2G:
2-acetamido-2-deoxy-alpha-D-galactopyranose

Source
  • gallus gallus (chicken)
KeywordsCELL ADHESION / FERTILIZATION / OOCYTE / EGG COAT / ZONA PELLUCIDA / VITELLINE ENVELOPE / ZP DOMAIN / ZP MODULE / EGG-SPERM INTERACTION / SPECIES-SPECIFIC GAMETE RECOGNITION / SPECIATION / BIODIVERSITY / INFERTILITY / EXTRACELLULAR MATRIX / IMMUNOGLOBULIN-LIKE FOLD / GLYCOPROTEIN / RECEPTOR / SECRETED / TRANSMEMBRANE / O-LINKED CARBOHYDRATE / T-ANTIGEN / CORE-1 / EXTERNAL HYDROPHOBIC PATCH / EHP / INTERNAL HYDROPHOBIC PATCH / IHP / SPERM-COMBINING SITE

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