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Structure paper

TitleNew Evidence for the Role of Calcium in the Glycosidase Reaction of Gh43 Arabinanases.
Journal, issue, pagesFEBS J., Vol. 277, Page 4562-, Year 2010
Publish dateMar 9, 2010 (structure data deposition date)
AuthorsDe Sanctis, D. / Inacio, J.M. / Lindley, P.F. / De Sa-Nogueira, I. / Bento, I.
External linksFEBS J. / PubMed:20883454
MethodsX-ray diffraction
Resolution1.5 - 1.9 Å
Structure data

PDB-2x8f:
Native structure of Endo-1,5-alpha-L-arabinanases from Bacillus subtilis
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-2x8s:
Crystal Structure of the Abn2 D171A mutant in complex with arabinotriose
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-2x8t:
Crystal Structure of the Abn2 H318A mutant
Method: X-RAY DIFFRACTION / Resolution: 1.79 Å

Chemicals

ChemComp-PO4:
PHOSPHATE ION

ChemComp-CA:
Unknown entry

ChemComp-GOL:
GLYCEROL

ChemComp-NA:
Unknown entry

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-MRD:
(4R)-2-METHYLPENTANE-2,4-DIOL / precipitant*YM

ChemComp-HOH:
WATER

ChemComp-CL:
Unknown entry

Source
  • bacillus subtilis (bacteria)
KeywordsHYDROLASE

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