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Structure paper

TitleConformational exchange in a membrane transport protein is altered in protein crystals.
Journal, issue, pagesBiophys. J., Vol. 99, Page 1604-1610, Year 2010
Publish dateMar 17, 2010 (structure data deposition date)
AuthorsFreed, D.M. / Horanyi, P.S. / Wiener, M.C. / Cafiso, D.S.
External linksBiophys. J. / PubMed:20816073
MethodsX-ray diffraction
Resolution2.44 Å
Structure data

PDB-3m8b:
Crystal structure of spin-labeled BtuB V10R1 in the apo state
Method: X-RAY DIFFRACTION / Resolution: 2.44 Å

PDB-3m8d:
Crystal structure of spin-labeled BtuB V10R1 with bound calcium and cyanocobalamin
Method: X-RAY DIFFRACTION / Resolution: 2.44 Å

Chemicals

ChemComp-C8E:
(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE / C8E, detergent*YM

ChemComp-MTN:
S-[(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl] methanesulfonothioate

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-CNC:
CYANOCOBALAMIN

ChemComp-CA:
Unknown entry

Source
  • escherichia coli (E. coli)
KeywordsTRANSPORT PROTEIN / beta barrel / R1 / spin label / Cell membrane / Cell outer membrane / Ion transport / Membrane / Metal-binding / Phage recognition / Porin / Receptor / TonB box / Transmembrane / Transport

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