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Structure paper

TitleFurther Insights Into the Roles of GTP and the C- Terminus of the Hepatitis C Virus Polymerase in the Initiation of RNA Synthesis
Journal, issue, pagesJ. Biol. Chem., Vol. 285, Page 32906-, Year 2010
Publish dateJun 21, 2010 (structure data deposition date)
AuthorsHarrus, D. / Ahmed-El-Sayed, N. / Simister, P.C. / Miller, S. / Triconnet, M. / Hagedorn, C.H. / Mahias, K. / Rey, F.A. / Astier-Gin, T. / Bressanelli, S.
External linksJ. Biol. Chem. / PubMed:20729191
MethodsX-ray diffraction
Resolution1.7 - 2.66 Å
Structure data

PDB-2xhu:
HCV-J4 NS5B Polymerase Orthorhombic Crystal Form
Method: X-RAY DIFFRACTION / Resolution: 2.287 Å

PDB-2xhv:
HCV-J4 NS5B Polymerase Point Mutant Orthorhombic Crystal Form
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-2xhw:
HCV-J4 NS5B Polymerase Trigonal Crystal Form
Method: X-RAY DIFFRACTION / Resolution: 2.66 Å

PDB-2xi2:
HCV-H77 NS5B Apo Polymerase
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-2xi3:
HCV-H77 NS5B Polymerase Complexed With GTP
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

ChemComp-MG:
Unknown entry

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

Source
  • hepatitis c virus genotype 1b (strain hc-j4)
  • hepatitis c virus genotype 1a (isolate h77)
  • hepatitis c virus (isolate h77)
KeywordsTRANSFERASE / REPLICATION / TRANSCRIPTION / NONSTRUCTURAL PROTEIN / RDRP / DE NOVO PRIMING / HEPACIVIRUS

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