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Structure paper

TitleStructures of DNA polymerases caught processing size-augmented nucleotide probes.
Journal, issue, pagesAngew. Chem. Int. Ed. Engl., Vol. 49, Page 5181-5184, Year 2010
Publish dateMar 19, 2010 (structure data deposition date)
AuthorsBetz, K. / Streckenbach, F. / Schnur, A. / Exner, T. / Welte, W. / Diederichs, K. / Marx, A.
External linksAngew. Chem. Int. Ed. Engl. / PubMed:20572212
MethodsX-ray diffraction
Resolution2 - 2.2 Å
Structure data

PDB-3m8r:
Crystal structure of the large fragment of DNA polymerase I from Thermus aquaticus in a closed ternary complex with trapped 4'-ethylated dTTP
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-3m8s:
Crystal structure of the large fragment of DNA polymerase I from Thermus aquaticus in a closed ternary complex with trapped 4'-methylated dTTP
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

Chemicals

ChemComp-HXZ:
4'-ethylthymidine 5'-(tetrahydrogen triphosphate)

ChemComp-MG:
Unknown entry

ChemComp-15P:
POLYETHYLENE GLYCOL (N=34) / precipitant*YM

ChemComp-GOL:
GLYCEROL

ChemComp-ACT:
ACETATE ION

ChemComp-HOH:
WATER

ChemComp-HXB:
4'-methylthymidine 5'-(tetrahydrogen triphosphate)

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

Source
  • thermus aquaticus (bacteria)
KeywordsTransferase/DNA / DNA / DNA polymerase / DNA replication / nucleotides / modified nucleotide / artificial nucleotide / nucleotide probes / DNA-binding / DNA-directed DNA polymerase / Nucleotidyltransferase / Transferase / Transferase-DNA complex

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