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Structure paper

TitleA distal point mutation in the streptavidin-biotin complex preserves structure but diminishes binding affinity: experimental evidence of electronic polarization effects?
Journal, issue, pagesBiochemistry, Vol. 49, Page 4568-4570, Year 2010
Publish dateApr 5, 2010 (structure data deposition date)
AuthorsBaugh, L. / Le Trong, I. / Cerutti, D.S. / Gulich, S. / Stayton, P.S. / Stenkamp, R.E. / Lybrand, T.P.
External linksBiochemistry / PubMed:20462252
MethodsX-ray diffraction
Resolution1.3 Å
Structure data

PDB-3mg5:
Core-streptavidin mutant F130L in complex with biotin
Method: X-RAY DIFFRACTION / Resolution: 1.3 Å

Chemicals

ChemComp-BTN:
BIOTIN

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

Source
  • streptomyces avidinii (bacteria)
KeywordsBIOTIN-BINDING PROTEIN / Streptavidin

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