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Structure paper

TitleComputational reprogramming of homing endonuclease specificity at multiple adjacent base pairs.
Journal, issue, pagesNucleic Acids Res., Vol. 38, Page 5601-5608, Year 2010
Publish dateNov 13, 2009 (structure data deposition date)
AuthorsAshworth, J. / Taylor, G.K. / Havranek, J.J. / Quadri, S.A. / Stoddard, B.L. / Baker, D.
External linksNucleic Acids Res. / PubMed:20435674
MethodsX-ray diffraction
Resolution2.3 - 2.9 Å
Structure data

PDB-3ko2:
I-MsoI re-designed for altered DNA cleavage specificity (-7C)
Method: X-RAY DIFFRACTION / Resolution: 2.9 Å

PDB-3mip:
I-MsoI re-designed for altered DNA cleavage specificity (-8GCG)
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-3mis:
I-MsoI re-designed for altered DNA cleavage specificity (-8G)
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-HOH:
WATER

Source
  • monomastix sp. (plant)
  • synthetic construct (others)
KeywordsHYDROLASE/DNA / redesign / protein-DNA complex / Chloroplast / Endonuclease / HYDROLASE-DNA complex / DE NOVO PROTEIN/DNA / homing nuclease / Rosetta design / DE NOVO PROTEIN-DNA complex

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