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Structure paper

TitleThe structures of the C185S and C185A mutants of sulfite oxidase reveal rearrangement of the active site.
Journal, issue, pagesBiochemistry, Vol. 49, Page 3989-4000, Year 2010
Publish dateMay 4, 2009 (structure data deposition date)
AuthorsQiu, J.A. / Wilson, H.L. / Pushie, M.J. / Kisker, C. / George, G.N. / Rajagopalan, K.V.
External linksBiochemistry / PubMed:20356030
MethodsX-ray diffraction
Resolution1.9 - 2.8 Å
Structure data

PDB-3hbg:
Structure of recombinant Chicken Liver Sulfite Oxidase mutant C185S
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-3hbp:
The crystal structure of C185S mutant of recombinant sulfite oxidase with bound substrate, sulfite, at the active site
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-3hbq:
Structure of recombinant Chicken Liver Sulfite Oxidase mutant Cys 185 Ala
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

Chemicals

ChemComp-MTE:
PHOSPHONIC ACIDMONO-(2-AMINO-5,6-DIMERCAPTO-4-OXO-3,7,8A,9,10,10A-HEXAHYDRO-4H-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-7-YLMETHYL)ESTER

ChemComp-MOM:
HYDROXY(DIOXO)MOLYBDENUM

ChemComp-HOH:
WATER

ChemComp-SO3:
SULFITE ION

ChemComp-GOL:
GLYCEROL

Source
  • gallus gallus (chicken)
KeywordsOXIDOREDUCTASE / sulfite oxidase / molybdenum / molybdopterin / oxotransferase / metal binding

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