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Structure paper

TitleProtein crystallography reveals a role for the FS0 cluster of Escherichia coli nitrate reductase A (NarGHI) in enzyme maturation.
Journal, issue, pagesJ. Biol. Chem., Vol. 285, Page 8801-8807, Year 2010
Publish dateAug 21, 2009 (structure data deposition date)
AuthorsRothery, R.A. / Bertero, M.G. / Spreter, T. / Bouromand, N. / Strynadka, N.C. / Weiner, J.H.
External linksJ. Biol. Chem. / PubMed:20053990
MethodsX-ray diffraction
Resolution2.3 - 2.8 Å
Structure data

PDB-3ir5:
Crystal structure of NarGHI mutant NarG-H49C
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-3ir6:
Crystal structure of NarGHI mutant NarG-H49S
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

PDB-3ir7:
Crystal structure of NarGHI mutant NarG-R94S
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

Chemicals

ChemComp-MD1:
PHOSPHORIC ACID 4-(2-AMINO-4-OXO-3,4,5,6,-TETRAHYDRO-PTERIDIN-6-YL)-2-HYDROXY-3,4-DIMERCAPTO-BUT-3-EN-YL ESTER GUANYLATE ESTER

ChemComp-6MO:
Unknown entry

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-AGA:
(1S)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PENTANOYLOXY)METHYL]ETHYL OCTANOATE

ChemComp-F3S:
FE3-S4 CLUSTER

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

ChemComp-HOH:
WATER

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

Source
  • escherichia coli k-12 (bacteria)
KeywordsOXIDOREDUCTASE / Nitrate reductase / electron transfer / membrane protein / 4Fe-4S / Cell membrane / Electron transport / Iron / Iron-sulfur / Membrane / Metal-binding / Molybdenum / Nitrate assimilation / Transport / 3Fe-4S / Cell inner membrane / Formylation / Heme / Transmembrane

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