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TitleMultiparameter screening on SlipChip used for nanoliter protein crystallization combining free interface diffusion and microbatch methods.
Journal, issue, pagesJ. Am. Chem. Soc., Vol. 132, Page 112-119, Year 2010
Publish dateNov 3, 2009 (structure data deposition date)
AuthorsLi, L. / Du, W. / Ismagilov, R.F.
External linksJ. Am. Chem. Soc. / PubMed:20000709
MethodsX-ray diffraction
Resolution1.95 Å
Structure data

PDB-3kjr:
Crystal structure of dihydrofolate reductase/thymidylate synthase from Babesia bovis determined using SlipChip based microfluidics
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

Chemicals

ChemComp-NAP:
NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

ChemComp-NHE:
2-[N-CYCLOHEXYLAMINO]ETHANE SULFONIC ACID / pH buffer*YM

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

Source
  • babesia bovis t2bo (eukaryote)
KeywordsTRANSFERASE / Microfluidics / SlipChip / Structural Genomics / PSI-2 / Protein Structure Initiative / Accelerated Technologies Center for Gene to 3D Structure / ATCG3D / Seattle Structural Genomics Center for Infectious Disease / Methyltransferase / SSGCID

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