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TitleA hydrogen-bonding network is important for oxidation and isomerization in the reaction catalyzed by cholesterol oxidase.
Journal, issue, pagesActa Crystallogr. ,Sect. D, Vol. 65, Page 1222-1231, Year 2009
Publish dateApr 3, 2009 (structure data deposition date)
AuthorsLyubimov, A.Y. / Chen, L. / Sampson, N.S. / Vrielink, A.
External linksActa Crystallogr. ,Sect. D / PubMed:19923719
MethodsX-ray diffraction
Resolution0.92 - 1 Å
Structure data

PDB-3gyi:
Cholesterol oxidase from Streptomyces sp. N485D mutant (1.0A)
Method: X-RAY DIFFRACTION / Resolution: 1 Å

PDB-3gyj:
Cholesterol oxidase from Streptomyces sp. N485L mutant (0.92A)
Method: X-RAY DIFFRACTION / Resolution: 0.92 Å

Chemicals

ChemComp-FAD:
FLAVIN-ADENINE DINUCLEOTIDE / FAD*YM

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

Source
  • streptomyces sp. (bacteria)
KeywordsOXIDOREDUCTASE / GMC oxidoreductase / site-directed mutagenesis / hydrophobic tunnel / flavoprotein / Cholesterol metabolism / FAD / Lipid metabolism / Secreted / Steroid metabolism

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