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TitlePractical Considerations When Using Temperature to Obtain Rate Constants and Activation Thermodynamics of Enzymes with Two Catalytic Steps: Native and N460T-beta-Galactosidase (E. coli) as Examples.
Journal, issue, pagesProtein J., Vol. 28, Page 96-103, Year 2009
Publish dateApr 29, 2008 (structure data deposition date)
AuthorsKappelhoff, J.C. / Liu, S.Y. / Dugdale, M.L. / Dymianiw, D.L. / Linton, L.R. / Huber, R.E.
External linksProtein J. / PubMed:19229596
MethodsX-ray diffraction
Resolution2.05 Å
Structure data

PDB-3czj:
E. COLI (lacZ) BETA-GALACTOSIDASE (N460T) IN COMPLEX WITH D-GALCTOPYRANOSYL-1-ONE
Method: X-RAY DIFFRACTION / Resolution: 2.05 Å

Chemicals

ChemComp-149:
D-galactonolactone

ChemComp-MG:
Unknown entry

ChemComp-NA:
Unknown entry

ChemComp-DMS:
DIMETHYL SULFOXIDE / DMSO, precipitant*YM

ChemComp-HOH:
WATER

Source
  • escherichia coli (E. coli)
KeywordsHYDROLASE / Asn-460-Thr BETA-GALACTOSIDASE HYDROLASE TIM BARREL (ALPHA/BETA BARREL) JELLY-ROLL BARREL IMMUNOGLOBULIN BETA SUPERSANDWICH / Glycosidase

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