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TitleJADAS: a customizable automated data acquisition system and its application to ice-embedded single particles.
Journal, issue, pagesJ Struct Biol, Vol. 165, Issue 1, Page 1-9, Year 2009
Publish dateSep 30, 2008
AuthorsJunjie Zhang / Natsuko Nakamura / Yuko Shimizu / Nathan Liang / Xiangan Liu / Joanita Jakana / Michael P Marsh / Christopher R Booth / Takao Shinkawa / Munetaka Nakata / Wah Chiu /
PubMed AbstractThe JEOL Automated Data Acquisition System (JADAS) is a software system built for the latest generation of the JEOL Transmission Electron Microscopes. It is designed to partially or fully automate ...The JEOL Automated Data Acquisition System (JADAS) is a software system built for the latest generation of the JEOL Transmission Electron Microscopes. It is designed to partially or fully automate image acquisition for ice-embedded single particles under low dose conditions. Its built-in flexibility permits users to customize the order of various imaging operations. In this paper, we describe how JADAS is used to accurately locate and image suitable specimen areas on a grid of ice-embedded particles. We also demonstrate the utility of JADAS by imaging the epsilon 15 bacteriophage with the JEM3200FSC electron cryo-microscope, showing that sufficient images can be collected in a single 8h session to yield a subnanometer resolution structure which agrees with the previously determined structure.
External linksJ Struct Biol / PubMed:18926912 / PubMed Central
MethodsEM (single particle)
Resolution7.3 Å
Structure data

EMDB-1557:
Cryo-EM density map of Epsilon-15 bacteriophage icosahedral reconstruction from JADAS automatically acquired data at 7.3 Angstrom resolution.
Method: EM (single particle) / Resolution: 7.3 Å

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