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Structure paper

TitleDNA distortion and specificity in a sequence-specific endonuclease.
Journal, issue, pagesJ. Mol. Biol., Vol. 383, Page 186-204, Year 2008
Publish dateAug 8, 2008 (structure data deposition date)
AuthorsBabic, A.C. / Little, E.J. / Manohar, V.M. / Bitinaite, J. / Horton, N.C.
External linksJ. Mol. Biol. / PubMed:18762194
MethodsX-ray diffraction
Resolution2.1 - 2.78 Å
Structure data

PDB-3e3y:
Q138F HincII bound to GTTAAC and cocrystallized with 5 mM Ca2+
Method: X-RAY DIFFRACTION / Resolution: 2.13 Å

PDB-3e40:
Q138F HincII bound to GTTAAC and cocrystallized with 5 mM Ca2+
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-3e41:
Q138F HincII bound to GTCGAC and 5 mM Ca2+
Method: X-RAY DIFFRACTION / Resolution: 2.73 Å

PDB-3e42:
Q138F HincII bound to GTCGAC and Ca2+ (cocrystallized)
Method: X-RAY DIFFRACTION / Resolution: 2.68 Å

PDB-3e43:
Q138F HincII bound to GTTAAC and cocrystallized with 2.5 mM MgCl2
Method: X-RAY DIFFRACTION / Resolution: 2.73 Å

PDB-3e44:
Q138F HincII bound to cleaved DNA (GTT | AAC) and Mn2+
Method: X-RAY DIFFRACTION / Resolution: 2.52 Å

PDB-3e45:
Q138F HincII bound to Noncognate DNA (GTGCAC) and Ca2+
Method: X-RAY DIFFRACTION / Resolution: 2.78 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-NA:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-UNX:
Unknown entry

ChemComp-MN:
Unknown entry

Source
  • haemophilus influenzae (bacteria)
KeywordsHYDROLASE/DNA / protein-DNA complex / endonuclease / indirect readout / Hydrolase / Nuclease / Restriction system / HYDROLASE-DNA COMPLEX / Protein-DNA Interaction / Restriction Endonuclease

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