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TitleBiochemical and structural characterisation of membrane-containing icosahedral dsDNA bacteriophages infecting thermophilic Thermus thermophilus.
Journal, issue, pagesVirology, Vol. 379, Issue 1, Page 10-19, Year 2008
Publish dateSep 15, 2008
AuthorsS T Jaatinen / L J Happonen / P Laurinmäki / S J Butcher / D H Bamford /
PubMed AbstractIcosahedral dsDNA viruses isolated from hot springs and proposed to belong to the Tectiviridae family infect the gram-negative thermophilic Thermus thermophilus bacterium. Seven such viruses were ...Icosahedral dsDNA viruses isolated from hot springs and proposed to belong to the Tectiviridae family infect the gram-negative thermophilic Thermus thermophilus bacterium. Seven such viruses were obtained from the Promega Corporation collection. The structural protein patterns of three of these viruses, growing to a high titer, appeared very similar but not identical. The most stable virus, P23-77, was chosen for more detailed studies. Analysis of highly purified P23-77 by thin layer chromatography for neutral lipids showed lipid association with the virion. Cryo-EM based three-dimensional image reconstruction of P23-77 to 1.4 nm resolution revealed an icosahedrally-ordered protein coat, with spikes on the vertices, and an internal membrane. The capsid architecture of P23-77 is most similar to that of the archaeal virus SH1. These findings further complicate the grouping of icosahedrally-symmetric viruses containing an inner membrane. We propose a single superfamily or order with members in several viral families.
External linksVirology / PubMed:18657283
MethodsEM (single particle)
Resolution14.0 Å
Structure data

EMDB-1525:
Structure of Thermusphage P23-77 using electron cryomicroscopy and three-dimensional image reconstruction
Method: EM (single particle) / Resolution: 14.0 Å

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