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Structure paper

TitleCrystal structures of I-SceI complexed to nicked DNA substrates: snapshots of intermediates along the DNA cleavage reaction pathway.
Journal, issue, pagesNucleic Acids Res., Vol. 36, Page 3287-3296, Year 2008
Publish dateJan 21, 2008 (structure data deposition date)
AuthorsMoure, C.M. / Gimble, F.S. / Quiocho, F.A.
External linksNucleic Acids Res. / PubMed:18424798
MethodsX-ray diffraction
Resolution2.2 - 2.3 Å
Structure data

PDB-3c0w:
I-SceI in complex with a bottom nicked DNA substrate
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-3c0x:
I-SceI in complex with a top nicked DNA substrate
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-NA:
Unknown entry

ChemComp-HOH:
WATER

Source
  • saccharomyces cerevisiae (brewer's yeast)
Keywordshydrolase/DNA / endonuclease / homing / LADLIDADG / catalytic mechanism / metal binding / nicked intermediate / Hydrolase / Intron homing / Mitochondrion / mRNA processing / mRNA splicing / hydrolase-DNA COMPLEX

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