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TitleBasis of altered RNA-binding specificity by PUF proteins revealed by crystal structures of yeast Puf4p
Journal, issue, pagesNat. Struct. Mol. Biol., Vol. 15, Page 397-402, Year 2008
Publish dateJan 10, 2008 (structure data deposition date)
AuthorsMiller, M.T. / Higgin, J.J. / Hall, T.M.T.
External linksNat. Struct. Mol. Biol. / PubMed:18327269
MethodsX-ray diffraction
Resolution2.69 - 3 Å
Structure data

PDB-3bwt:
Crystal structure of the RNA binding domain of Puf4 from Saccharomyces cerevisiae
Method: X-RAY DIFFRACTION / Resolution: 2.69 Å

PDB-3bx2:
Puf4 RNA binding domain bound to HO endonuclease RNA 3' UTR recognition sequence
Method: X-RAY DIFFRACTION / Resolution: 2.84 Å

PDB-3bx3:
Puf4 T650C/C724R Mutant bound to Cox17 RNA 3' UTR recognition sequence
Method: X-RAY DIFFRACTION / Resolution: 3 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-SO4:
SULFATE ION

ChemComp-NA:
Unknown entry

Source
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsTRANSCRIPTION / RNA BINDING PROTEIN / Puf4 / Pumilio / RNA Binding / HO endonuclease / RNA BINDING PROTEIN/RNA / RNA BINDING PROTEIN-RNA COMPLEX / Puf4 T650C C724R mutant

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