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Structure paper

TitleInhibition kinetics and emodin cocrystal structure of a type II polyketide ketoreductase
Journal, issue, pagesBiochemistry, Vol. 47, Page 1837-1847, Year 2008
Publish dateOct 5, 2007 (structure data deposition date)
AuthorsKorman, T.P. / Tan, Y.H. / Wong, J. / Luo, R. / Tsai, S.-C.
External linksBiochemistry / PubMed:18205400
MethodsX-ray diffraction
Resolution2.1 - 2.5 Å
Structure data

PDB-2rh4:
Actinorhodin ketoreductase, actKR, with NADPH and Inhibitor Emodin
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-2rhc:
Actinorhodin ketordeuctase, actKR, with NADP+ and Inhibitor Emodin
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-2rhr:
P94L actinorhodin ketordeuctase mutant, with NADPH and Inhibitor Emodin
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

Chemicals

ChemComp-NDP:
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

ChemComp-EMO:
3-METHYL-1,6,8-TRIHYDROXYANTHRAQUINONE

ChemComp-HOH:
WATER

ChemComp-NAP:
NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

Source
  • streptomyces coelicolor (bacteria)
KeywordsOXIDOREDUCTASE / polyketide / actinorhodin / ketoreductase / combinatorial biosynthesis / short chain dehydrogenase/reductase

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