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TitleStructural determinants of nitroxide motion in spin-labeled proteins: Solvent-exposed sites in helix B of T4 lysozyme.
Journal, issue, pagesProtein Sci., Vol. 17, Page 228-239, Year 2008
Publish dateJun 12, 2007 (structure data deposition date)
AuthorsGuo, Z. / Cascio, D. / Hideg, K. / Hubbell, W.L.
External linksProtein Sci. / PubMed:18096642
MethodsX-ray diffraction / EPR
Resolution1.4 - 2.1 Å
Structure data

PDB-2q9d:
Structure of spin-labeled T4 lysozyme mutant A41R1
Method: X-RAY DIFFRACTION / EPR / Resolution: 1.4 Å

PDB-2q9e:
Structure of spin-labeled T4 lysozyme mutant S44R1
Method: X-RAY DIFFRACTION / EPR / Resolution: 2.1 Å

Chemicals

ChemComp-MTN:
S-[(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl] methanesulfonothioate

ChemComp-BME:
BETA-MERCAPTOETHANOL

ChemComp-HOH:
WATER

ChemComp-HED:
2-HYDROXYETHYL DISULFIDE

Source
  • enterobacteria phage t4 (virus)
KeywordsHYDROLASE / Nitroxide / Spin label / T4 lysozyme / Electron paramagnetic resonance / EPR

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