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TitleStructural and thermodynamic basis for enhanced DNA binding by a promiscuous mutant EcoRI endonuclease.
Journal, issue, pagesStructure, Vol. 15, Page 1368-1382, Year 2007
Publish dateFeb 21, 2007 (structure data deposition date)
AuthorsSapienza, P.J. / Rosenberg, J.M. / Jen-Jacobson, L.
External linksStructure / PubMed:17997963
MethodsX-ray diffraction
Resolution1.95 Å
Structure data

PDB-2oxv:
Structure of the A138T promiscuous mutant of the EcoRI restriction endonuclease bound to its cognate recognition site.
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

Chemicals

ChemComp-HOH:
WATER

Source
  • escherichia coli (E. coli)
Keywordshydrolase/DNA / EcoRI / type II restriction endonuclease / protein-DNA interactions / promiscuous mutant / relaxed specificity mutant / hydrolase-DNA COMPLEX

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