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TitleUltrafast excited-state dynamics in the green fluorescent protein variant S65T/H148D. 1. Mutagenesis and structural studies.
Journal, issue, pagesBiochemistry, Vol. 46, Page 12005-12013, Year 2007
Publish dateJul 23, 2006 (structure data deposition date)
AuthorsShu, X. / Kallio, K. / Shi, X. / Abbyad, P. / Kanchanawong, P. / Childs, W. / Boxer, S.G. / Remington, S.J.
External linksBiochemistry / PubMed:17918959
MethodsX-ray diffraction
Resolution1.2 - 1.5 Å
Structure data

PDB-2due:
crystal structure of a green fluorescent protein variant S65T/H148D at pH 10
Method: X-RAY DIFFRACTION / Resolution: 1.24 Å

PDB-2duf:
crystal structure of a green fluorescent protein variant S65T/H148D at pH 5.6
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-2dug:
crystal structure of a green fluorescent protein S65T/H148N at pH 5
Method: X-RAY DIFFRACTION / Resolution: 1.4 Å

PDB-2duh:
crystal structure of a green fluorescent protein variant S65T/H148N at pH 9.5
Method: X-RAY DIFFRACTION / Resolution: 1.2 Å

PDB-2dui:
crystal structure of a green fluorescent protein variant H148D at pH 9
Method: X-RAY DIFFRACTION / Resolution: 1.36 Å

Chemicals

ChemComp-HOH:
WATER

Source
  • aequorea victoria (jellyfish)
KeywordsLUMINESCENT PROTEIN / excited state proton transfer / very short hydrogen bond / green fluorescent protein

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