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Title2-Aminopurine Flipped into the Active Site of the Adenine-Specific DNA Methyltransferase M.TaqI: Crystal Structures and Time-Resolved Fluorescence
Journal, issue, pagesJ. Am. Chem. Soc., Vol. 129, Page 6240-6248, Year 2007
Publish dateSep 12, 2006 (structure data deposition date)
AuthorsLenz, T. / Bonnist, E.Y.M. / Pljevaljcic, G. / Neely, R.K. / Dryden, D.T.F. / Scheidig, A.J. / Jones, A.C. / Weinhold, E.
External linksJ. Am. Chem. Soc. / PubMed:17455934
MethodsX-ray diffraction
Resolution1.7 - 2.4 Å
Structure data

PDB-2ibs:
Crystal structure of the adenine-specific DNA methyltransferase M.TaqI complexed with the cofactor analog AETA and a 10 bp DNA containing 2-aminopurine at the target position
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-2ibt:
Crystal structure of the adenine-specific DNA methyltransferase M.TaqI complexed with the cofactor analog AETA and a 10 bp DNA containing 2-aminopurine at the target position and an abasic site analog at the target base partner position
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

Chemicals

ChemComp-NEA:
5'-DEOXY-5'-[2-(AMINO)ETHYLTHIO]ADENOSINE

ChemComp-HOH:
WATER

ChemComp-GOL:
GLYCEROL

Source
  • thermus aquaticus (bacteria)
KeywordsTRANSFERASE/DNA / DNA / DNA methyltransferase / 2-aminopurine / base flipping / nucleotide flipping / TRANSFERASE-DNA COMPLEX / abasic site analog

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