+検索条件
-Structure paper
| タイトル | Insights into transcription initiation and termination from the electron microscopy structure of yeast RNA polymerase III. |
|---|---|
| ジャーナル・号・ページ | Mol Cell, Vol. 25, Issue 6, Page 813-823, Year 2007 |
| 掲載日 | 2007年3月23日 |
著者 | Carlos Fernández-Tornero / Bettina Böttcher / Michel Riva / Christophe Carles / Ulrich Steuerwald / Rob W H Ruigrok / André Sentenac / Christoph W Müller / Guy Schoehn / ![]() |
| PubMed 要旨 | RNA polymerase III (RNAPIII) synthesizes tRNA, 5S RNA, U6 snRNA, and other small RNAs. The structure of yeast RNAPIII, determined at 17 A resolution by cryo-electron microscopy and single-particle ...RNA polymerase III (RNAPIII) synthesizes tRNA, 5S RNA, U6 snRNA, and other small RNAs. The structure of yeast RNAPIII, determined at 17 A resolution by cryo-electron microscopy and single-particle analysis, reveals a hand-like shape typical of RNA polymerases. Compared to RNAPII, RNAPIII is characterized by a bulkier stalk and by prominent features extending from the DNA binding cleft. We attribute the latter primarily to five RNAPIII-specific subunits, present as two distinct subcomplexes (C82/C34/C31 and C53/C37). Antibody labeling experiments localize the C82/C34/C31 subcomplex to the clamp side of the DNA binding cleft, consistent with its known role in transcription initiation. The C53/C37 subcomplex appears to be situated across the cleft, near the presumed location of downstream DNA, accounting for its role in transcription termination. Our structure rationalizes available mutagenesis and biochemical data and provides insights into RNAPIII-mediated transcription. |
リンク | Mol Cell / PubMed:17386259 |
| 手法 | EM (単粒子) |
| 解像度 | 17.0 Å |
| 構造データ | ![]() EMDB-1322: |
| 由来 |
|
ムービー
コントローラー
構造ビューア
万見文献について



著者
リンク

