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TitleInsights into transcription initiation and termination from the electron microscopy structure of yeast RNA polymerase III.
Journal, issue, pagesMol Cell, Vol. 25, Issue 6, Page 813-823, Year 2007
Publish dateMar 23, 2007
AuthorsCarlos Fernández-Tornero / Bettina Böttcher / Michel Riva / Christophe Carles / Ulrich Steuerwald / Rob W H Ruigrok / André Sentenac / Christoph W Müller / Guy Schoehn /
PubMed AbstractRNA polymerase III (RNAPIII) synthesizes tRNA, 5S RNA, U6 snRNA, and other small RNAs. The structure of yeast RNAPIII, determined at 17 A resolution by cryo-electron microscopy and single-particle ...RNA polymerase III (RNAPIII) synthesizes tRNA, 5S RNA, U6 snRNA, and other small RNAs. The structure of yeast RNAPIII, determined at 17 A resolution by cryo-electron microscopy and single-particle analysis, reveals a hand-like shape typical of RNA polymerases. Compared to RNAPII, RNAPIII is characterized by a bulkier stalk and by prominent features extending from the DNA binding cleft. We attribute the latter primarily to five RNAPIII-specific subunits, present as two distinct subcomplexes (C82/C34/C31 and C53/C37). Antibody labeling experiments localize the C82/C34/C31 subcomplex to the clamp side of the DNA binding cleft, consistent with its known role in transcription initiation. The C53/C37 subcomplex appears to be situated across the cleft, near the presumed location of downstream DNA, accounting for its role in transcription termination. Our structure rationalizes available mutagenesis and biochemical data and provides insights into RNAPIII-mediated transcription.
External linksMol Cell / PubMed:17386259
MethodsEM (single particle)
Resolution17.0 Å
Structure data

EMDB-1322:
Insights into transcription initiation and termination from the electron microscopy structure of yeast RNA polymerase III.
Method: EM (single particle) / Resolution: 17.0 Å

Source
  • Saccharomyces cerevisiae (brewer's yeast)

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