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TitleStructural and Mechanistic Analyses of endo-Glycoceramidase II, a Membrane-associated Family 5 Glycosidase in the Apo and GM3 Ganglioside-bound Forms.
Journal, issue, pagesJ. Biol. Chem., Vol. 282, Page 14300-14308, Year 2007
Publish dateFeb 6, 2007 (structure data deposition date)
AuthorsCaines, M.E. / Vaughan, M.D. / Tarling, C.A. / Hancock, S.M. / Warren, R.A. / Withers, S.G. / Strynadka, N.C.
External linksJ. Biol. Chem. / PubMed:17329247
MethodsX-ray diffraction
Resolution1.1 - 2.1 Å
Structure data

PDB-2osw:
Endo-glycoceramidase II from Rhodococcus sp.
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-2osx:
Endo-glycoceramidase II from Rhodococcus sp.: Ganglioside GM3 Complex
Method: X-RAY DIFFRACTION / Resolution: 1.1 Å

PDB-2osy:
Endo-glycoceramidase II from Rhodococcus sp.: Lactosyl-Enzyme Intermediate
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

Chemicals

ChemComp-NA:
Unknown entry

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-HOH:
WATER

ChemComp-16C:
N-((E,2S,3R)-1,3-DIHYDROXYOCTADEC-4-EN-2-YL)PALMITAMIDE

ChemComp-GOL:
GLYCEROL

Source
  • rhodococcus sp. (bacteria)
KeywordsHYDROLASE / (alpha/beta)8 (TIM) barrel

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