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TitleX-ray, NMR, and mutational studies of the catalytic cycle of the GDP-mannose mannosyl hydrolase reaction.
Journal, issue, pagesBiochemistry, Vol. 45, Page 11290-11303, Year 2006
Publish dateApr 27, 2006 (structure data deposition date)
AuthorsGabelli, S.B. / Azurmendi, H.F. / Bianchet, M.A. / Amzel, L.M. / Mildvan, A.S.
External linksBiochemistry / PubMed:16981689
MethodsX-ray diffraction
Resolution2 - 2.3 Å
Structure data

PDB-2gt2:
Structure of the E. coli GDP-mannose mannosyl hydrolase
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-2gt4:
Crystal Structure of the Y103F mutant of the GDP-mannose mannosyl hydrolase in complex with GDP-mannose and MG+2
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-MG:
Unknown entry

ChemComp-GDD:
GUANOSINE-5'-DIPHOSPHATE-ALPHA-D-MANNOSE

ChemComp-BMA:
beta-D-mannopyranose

Source
  • escherichia coli (E. coli)
  • escherichia coli k12 (bacteria)
KeywordsHYDROLASE / GDP-mannose hydrolase GDP-glucose hydrolase NUDIX / GDP-mannose hydrolase GDP-glucose hydrolase NUDIX GDP GDP-fucose

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