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-Structure paper
Title | Cryo-electron tomographic structure of an immunodeficiency virus envelope complex in situ. |
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Journal, issue, pages | PLoS Pathog, Vol. 2, Issue 8, Page e83, Year 2006 |
Publish date | Nov 30, 2006 |
![]() | Giulia Zanetti / John A G Briggs / Kay Grünewald / Quentin J Sattentau / Stephen D Fuller / ![]() |
PubMed Abstract | The envelope glycoprotein (Env) complexes of the human and simian immunodeficiency viruses (HIV and SIV, respectively) mediate viral entry and are a target for neutralizing antibodies. The receptor ...The envelope glycoprotein (Env) complexes of the human and simian immunodeficiency viruses (HIV and SIV, respectively) mediate viral entry and are a target for neutralizing antibodies. The receptor binding surfaces of Env are in large part sterically occluded or conformationally masked prior to receptor binding. Knowledge of the unliganded, trimeric Env structure is key for an understanding of viral entry and immune escape, and for the design of vaccines to elicit neutralizing antibodies. We have used cryo-electron tomography and averaging to obtain the structure of the SIV Env complex prior to fusion. Our result reveals novel details of Env organisation, including tight interaction between monomers in the gp41 trimer, associated with a three-lobed, membrane-distal gp120 trimer. A cavity exists at the gp41-gp120 trimer interface. Our model for the spike structure agrees with previously predicted interactions between gp41 monomers, and furthers our understanding of gp120 interactions within an intact spike. |
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Methods | EM (subtomogram averaging) |
Resolution | 28.0 Å |
Structure data | ![]() EMDB-1216: |
Source |
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