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TitleSubstrate specificity in enzymatic fluorination. The fluorinase from Streptomyces cattleya accepts 2'-deoxyadenosine substrates.
Journal, issue, pagesOrg. Biomol. Chem., Vol. 4, Page 1458-1460, Year 2006
Publish dateOct 22, 2005 (structure data deposition date)
AuthorsCobb, S.L. / Deng, H. / McEwan, A.R. / Naismith, J.H. / O'Hagan, D. / Robinson, D.A.
External linksOrg. Biomol. Chem. / PubMed:16604208
MethodsX-ray diffraction
Resolution2 - 2.5 Å
Structure data

PDB-2c4u:
Crystal structure of the apo form of the 5'-Fluoro-5'-deoxyadenosine synthase enzyme from Streptomyces cattleya
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-2c5b:
X-ray crystal structure of 5'-fluorodeoxyadenosine synthase from Streptomyces cattleya complexed with 2'deoxy-5'deoxy-fluoroadenosine.
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-2cbx:
X-ray crystal structure of 5'-fluorodeoxyadenosine synthase from Streptomyces cattleya complexed with beta-D-erythrofuranosyl- adenosine
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-2cc2:
X-ray crystal structure of 5'-fluorodeoxyadenosine synthase from Streptomyces cattleya complexed with 5'deoxyadenosine
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-MET:
METHIONINE

ChemComp-5F1:
5'-FLUORO-2',5'-DIDEOXYADENOSINE

ChemComp-CC5:
BETA-D-ERYTHROFURANOSYL-ADENOSINE

ChemComp-5AD:
5'-DEOXYADENOSINE

ChemComp-CL:
Unknown entry

Source
  • streptomyces cattleya (bacteria)
KeywordsTRANSFERASE / FLUORINASE / APO / SAM / FDA / 5'-FLUORODEOXYADANOSINE SYNTHASE / 5'-FLUORODEOXYADENOSINE SYNTHASE / FLA / INHIBITOR / AZA / STREPTOMYCES CATTLEYA / SAM ANALOGUE / 5'DEOXYADENOSINE

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