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TitleA double mutation of Escherichia coli2C-methyl-D-erythritol-2,4-cyclodiphosphate synthase disrupts six hydrogen bonds with, yet fails to prevent binding of, an isoprenoid diphosphate.
Journal, issue, pagesActa Crystallogr. ,Sect. F, Vol. 61, Page 625-629, Year 2005
Publish dateFeb 2, 2005 (structure data deposition date)
AuthorsSgraja, T. / Kemp, L.E. / Ramsden, N. / Hunter, W.N.
External linksActa Crystallogr. ,Sect. F / PubMed:16511114
MethodsX-ray diffraction
Resolution3.11 Å
Structure data

PDB-1yqn:
E. coli ispF double mutant
Method: X-RAY DIFFRACTION / Resolution: 3.11 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MN:
Unknown entry

ChemComp-CDP:
CYTIDINE-5'-DIPHOSPHATE

ChemComp-GPP:
GERANYL DIPHOSPHATE

ChemComp-HOH:
WATER

Source
  • escherichia coli (E. coli)
KeywordsLYASE / 2C-methyl-D-erythriol-2 / 4-cyclodiphosphate synthase R142M/E144K (ispF mutant) / alpha-beta protein

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