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-Structure paper
タイトル | Structure of epsilon15 bacteriophage reveals genome organization and DNA packaging/injection apparatus. |
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ジャーナル・号・ページ | Nature, Vol. 439, Issue 7076, Page 612-616, Year 2006 |
掲載日 | 2006年2月2日 |
著者 | Wen Jiang / Juan Chang / Joanita Jakana / Peter Weigele / Jonathan King / Wah Chiu / |
PubMed 要旨 | The critical viral components for packaging DNA, recognizing and binding to host cells, and injecting the condensed DNA into the host are organized at a single vertex of many icosahedral viruses. ...The critical viral components for packaging DNA, recognizing and binding to host cells, and injecting the condensed DNA into the host are organized at a single vertex of many icosahedral viruses. These component structures do not share icosahedral symmetry and cannot be resolved using a conventional icosahedral averaging method. Here we report the structure of the entire infectious Salmonella bacteriophage epsilon15 (ref. 1) determined from single-particle cryo-electron microscopy, without icosahedral averaging. This structure displays not only the icosahedral shell of 60 hexamers and 11 pentamers, but also the non-icosahedral components at one pentameric vertex. The densities at this vertex can be identified as the 12-subunit portal complex sandwiched between an internal cylindrical core and an external tail hub connecting to six projecting trimeric tailspikes. The viral genome is packed as coaxial coils in at least three outer layers with approximately 90 terminal nucleotides extending through the protein core and the portal complex and poised for injection. The shell protein from icosahedral reconstruction at higher resolution exhibits a similar fold to that of other double-stranded DNA viruses including herpesvirus, suggesting a common ancestor among these diverse viruses. The image reconstruction approach should be applicable to studying other biological nanomachines with components of mixed symmetries. |
リンク | Nature / PubMed:16452981 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 9.5 - 20.0 Å |
構造データ | EMDB-1175: EMDB-1176: |
由来 |
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