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Structure paper

TitleStructure of the Metal-Independent Restriction Enzyme Bfii Reveals Fusion of a Specific DNA-Binding Domain with a Nonspecific Nuclease.
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Vol. 102, Page 15797-, Year 2005
Publish dateSep 15, 2005 (structure data deposition date)
AuthorsGrazulis, S. / Manakova, E. / Roessle, M. / Bochtler, M. / Tamulaitiene, G. / Huber, R. / Siksnys, V.
External linksProc. Natl. Acad. Sci. USA / PubMed:16247004
MethodsX-ray diffraction
Resolution1.9 Å
Structure data

PDB-2c1l:
Structure of the BfiI restriction endonuclease
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-TLA:
L(+)-TARTARIC ACID

ChemComp-SRT:
S,R MESO-TARTARIC ACID

ChemComp-TAR:
D(-)-TARTARIC ACID

ChemComp-BCT:
BICARBONATE ION / pH buffer*YM

ChemComp-MES:
2-(N-MORPHOLINO)-ETHANESULFONIC ACID / pH buffer*YM

ChemComp-HOH:
WATER

Source
  • bacillus firmus (bacteria)
KeywordsHYDROLASE / BFII / RESTRICTION ENDONUCLEASE / DOMAIN FUSION

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