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-Structure paper
タイトル | ATPase-dependent cooperative binding of ORC and Cdc6 to origin DNA. |
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ジャーナル・号・ページ | Nat Struct Mol Biol, Vol. 12, Issue 11, Page 965-971, Year 2005 |
掲載日 | 2006年5月31日 |
![]() | Christian Speck / Zhiqiang Chen / Huilin Li / Bruce Stillman / ![]() |
PubMed 要旨 | Binding of Cdc6 to the origin recognition complex (ORC) is a key step in the assembly of a pre-replication complex (pre-RC) at origins of DNA replication. ORC recognizes specific origin DNA sequences ...Binding of Cdc6 to the origin recognition complex (ORC) is a key step in the assembly of a pre-replication complex (pre-RC) at origins of DNA replication. ORC recognizes specific origin DNA sequences in an ATP-dependent manner. Here we demonstrate cooperative binding of Saccharomyces cerevisiae Cdc6 to ORC on DNA in an ATP-dependent manner, which induces a change in the pattern of origin binding that requires the Orc1 ATPase. The reaction is blocked by specific origin mutations that do not interfere with the interaction between ORC and DNA. Single-particle reconstruction of electron microscopic images shows that the ORC-Cdc6 complex forms a ring-shaped structure with dimensions similar to those of the ring-shaped MCM helicase. The ORC-Cdc6 structure is predicted to contain six AAA+ subunits, analogous to other ATP-dependent protein machines. We suggest that Cdc6 and origin DNA activate a molecular switch in ORC that contributes to pre-RC assembly. |
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手法 | EM (単粒子) |
解像度 | 20.0 Å |
構造データ | ![]() EMDB-1156: ![]() EMDB-1157: |
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