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TitleOxidative Chemistry in the GFP Active Site Leads to Covalent Cross-Linking of a Modified Leucine Side Chain with a Histidine Imidazole: Implications for the Mechanism of Chromophore Formation.
Journal, issue, pagesBiochemistry, Vol. 44, Page 8303-8311, Year 2005
Publish dateMar 4, 2005 (structure data deposition date)
AuthorsRosenow, M.A. / Patel, H.N. / Wachter, R.M.
External linksBiochemistry / PubMed:15938620
MethodsX-ray diffraction
Resolution1.5 - 2 Å
Structure data

PDB-1z1p:
Y66L variant of Enhanced Green Fluorescent Protein with 412-nm Absorbing Chromophore
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-1z1q:
Y66L Variant of Enhanced Green Fluorescent Protein with 374-nm Absorbing Chromophore
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-NA:
Unknown entry

Source
  • aequorea victoria (jellyfish)
KeywordsLUMINESCENT PROTEIN / GFP / Beta Barrel / UV/Vis absorbing yellow chromophore / Covalent cross-link

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