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Structure paper

TitleAlanine-scanning mutagenesis of the beta-sheet region of phage T4 lysozyme suggests that tertiary context has a dominant effect on beta-sheet formation
Journal, issue, pagesProtein Sci., Vol. 13, Page 2716-2724, Year 2004
Publish dateMar 24, 2004 (structure data deposition date)
AuthorsHe, M.M. / Wood, Z.A. / Baase, W.A. / Xiao, H. / Matthews, B.W.
External linksProtein Sci. / PubMed:15340171
MethodsX-ray diffraction
Resolution1.8 - 2.4 Å
Structure data

PDB-1ssw:
Crystal structure of phage T4 lysozyme mutant Y24A/Y25A/T26A/I27A/C54T/C97A
Method: X-RAY DIFFRACTION / Resolution: 2.13 Å

PDB-1ssy:
Crystal structure of phage T4 lysozyme mutant G28A/I29A/G30A/C54T/C97A
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-1t8f:
Crystal structure of phage T4 lysozyme mutant R14A/K16A/I17A/K19A/T21A/E22A/C54T/C97A
Method: X-RAY DIFFRACTION / Resolution: 2.15 Å

PDB-1t8g:
Crystal structure of phage T4 lysozyme mutant L32A/L33A/T34A/C54T/C97A/E108V
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

Chemicals

ChemComp-BME:
BETA-MERCAPTOETHANOL

ChemComp-HOH:
WATER

ChemComp-CL:
Unknown entry

ChemComp-PO4:
PHOSPHATE ION

Source
  • enterobacteria phage t4 (virus)
KeywordsHYDROLASE / Glycosidase / Bacteriolytic enzyme / poly-alanine mutation / T4 / lysozyme / poly-alanine

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