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Structure paper

TitleCrystal structure of the Mycobacterium tuberculosis dUTPase: insights into the catalytic mechanism.
Journal, issue, pagesJ. Mol. Biol., Vol. 341, Page 503-517, Year 2004
Publish dateSep 13, 2002 (structure data deposition date)
AuthorsChan, S. / Segelke, B. / Lekin, T. / Krupka, H. / Cho, U.S. / Kim, M.-Y. / So, M. / Kim, C.-Y. / Naranjo, C.M. / Rogers, Y.C. ...Chan, S. / Segelke, B. / Lekin, T. / Krupka, H. / Cho, U.S. / Kim, M.-Y. / So, M. / Kim, C.-Y. / Naranjo, C.M. / Rogers, Y.C. / Park, M.S. / Waldo, G.S. / Pashkov, I. / Cascio, D. / Perry, J.L. / Sawaya, M.R.
External linksJ. Mol. Biol. / PubMed:15276840
MethodsX-ray diffraction
Resolution1.3 - 3 Å
Structure data

PDB-1mq7:
CRYSTAL STRUCTURE OF DUTPASE FROM MYCOBACTERIUM TUBERCULOSIS (RV2697C)
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

PDB-1six:
Mycobacterium tuberculosis dUTPase complexed with magnesium and alpha,beta-imido-dUTP
Method: X-RAY DIFFRACTION / Resolution: 1.3 Å

PDB-1sjn:
Mycobacterium tuberculosis dUTPase complexed with magnesium and alpha,beta-imido-dUTP
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-1slh:
Mycobacterium tuberculosis dUTPase complexed with magnesium and dUDP
Method: X-RAY DIFFRACTION / Resolution: 3 Å

PDB-1sm8:
M. tuberculosis dUTPase complexed with chromium and dUTP
Method: X-RAY DIFFRACTION / Resolution: 2.9 Å

PDB-1smc:
Mycobacterium tuberculosis dUTPase complexed with dUTP in the absence of metal ion.
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-1snf:
MYCOBACTERIUM TUBERCULOSIS DUTPASE COMPLEXED WITH MAGNESIUM AND DEOXYURIDINE 5'-MONOPHOSPHATE
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

Chemicals

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-HOH:
WATER

ChemComp-MG:
Unknown entry

ChemComp-DUP:
2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-TRIPHOSPHATE

ChemComp-NO3:
NITRATE ION

ChemComp-DUD:
DEOXYURIDINE-5'-DIPHOSPHATE

ChemComp-CR:
CHROMIUM ION

ChemComp-DUT:
DEOXYURIDINE-5'-TRIPHOSPHATE

ChemComp-UMP:
2'-DEOXYURIDINE 5'-MONOPHOSPHATE

Source
  • mycobacterium tuberculosis (bacteria)
KeywordsHYDROLASE / jelly-roll / Structural Genomics / PSI / Protein Structure Initiative / TB Structural Genomics Consortium / TBSGC

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