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TitleCoenzyme Site-directed Mutants of Photosynthetic A(4)-GAPDH Show Selectively Reduced NADPH-dependent Catalysis, Similar to Regulatory AB-GAPDH Inhibited by Oxidized Thioredoxin
Journal, issue, pagesJ. Mol. Biol., Vol. 340, Page 1025-1037, Year 2004
Publish dateNov 27, 2003 (structure data deposition date)
AuthorsSparla, F. / Fermani, S. / Falini, G. / Zaffagnini, M. / Ripamonti, A. / Sabatino, P. / Pupillo, P. / Trost, P.
External linksJ. Mol. Biol. / PubMed:15236965
MethodsX-ray diffraction
Resolution2 - 2.2 Å
Structure data

PDB-1rm3:
Crystal structure of mutant T33A of photosynthetic glyceraldehyde-3-phosphate dehydrogenase A4 isoform, complexed with NADP
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-1rm4:
Crystal structure of recombinant photosynthetic glyceraldehyde-3-phosphate dehydrogenase A4 isoform, complexed with NADP
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-1rm5:
Crystal structure of mutant S188A of photosynthetic glyceraldehyde-3-phosphate dehydrogenase A4 isoform, complexed with NADP
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-NDP:
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

ChemComp-HOH:
WATER

Source
  • spinacia oleracea (spinach)
KeywordsOXIDOREDUCTASE / Rossmann fold / GAPDH-NADP complex

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