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TitleLigand-induced Conformational Changes and a Reaction Intermediate in Branched-chain 2-Oxo Acid Dehydrogenase (E1) from Thermus thermophilus HB8, as Revealed by X-ray Crystallography
Journal, issue, pagesJ. Mol. Biol., Vol. 337, Page 1011-1033, Year 2004
Publish dateSep 25, 2003 (structure data deposition date)
AuthorsNakai, T. / Nakagawa, N. / Maoka, N. / Masui, R. / Kuramitsu, S. / Kamiya, N.
External linksJ. Mol. Biol. / PubMed:15033367
MethodsX-ray diffraction
Resolution1.9 - 2.4 Å
Structure data

PDB-1um9:
branched-chain 2-oxo acid dehydrogenase (E1) from Thermus thermophilus HB8 in apo-form
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-1umb:
branched-chain 2-oxo acid dehydrogenase (E1) from Thermus thermophilus HB8 in holo-form
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-1umc:
branched-chain 2-oxo acid dehydrogenase (E1) from Thermus thermophilus HB8 with 4-methylpentanoate
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-1umd:
branched-chain 2-oxo acid dehydrogenase (E1) from Thermus thermophilus HB8 with 4-methyl-2-oxopentanoate as an intermediate
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

ChemComp-MG:
Unknown entry

ChemComp-TPP:
THIAMINE DIPHOSPHATE

ChemComp-4MV:
4-METHYL VALERIC ACID

ChemComp-COI:
2-OXO-4-METHYLPENTANOIC ACID

Source
  • thermus thermophilus (bacteria)
KeywordsOXIDOREDUCTASE / alpha(2)beta(2) tetramer / structural genomics / RIKEN Structural Genomics/Proteomics Initiative / RSGI

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