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TitleElectron cryomicroscopy structure of N-ethyl maleimide sensitive factor at 11 A resolution.
Journal, issue, pagesEMBO J, Vol. 22, Issue 17, Page 4365-4374, Year 2003
Publish dateSep 1, 2003
AuthorsJohannes Furst / R Bryan Sutton / James Chen / Axel T Brunger / Nikolaus Grigorieff /
PubMed AbstractN-ethyl maleimide sensitive factor (NSF) belongs to the AAA family of ATPases and is involved in a number of cellular functions, including vesicle fusion and trafficking of membrane proteins. We ...N-ethyl maleimide sensitive factor (NSF) belongs to the AAA family of ATPases and is involved in a number of cellular functions, including vesicle fusion and trafficking of membrane proteins. We present the three-dimensional structure of the hydrolysis mutant E329Q of NSF complexed with an ATP-ADP mixture at 11 A resolution by electron cryomicroscopy and single-particle averaging of NSF.alpha-SNAP.SNARE complexes. The NSF domains D1 and D2 form hexameric rings that are arranged in a double-layered barrel. Our structure is more consistent with an antiparallel orientation of the two rings rather than a parallel one. The crystal structure of the D2 domain of NSF was docked into the EM density map and shows good agreement, including details at the secondary structural level. Six protrusions corresponding to the N domain of NSF (NSF-N) emerge from the sides of the D1 domain ring. The density corresponding to alpha-SNAP and SNAREs is located on the 6-fold axis of the structure, near the NSF-N domains. The density of the N domain is weak, suggesting conformational variability in this part of NSF.
External linksEMBO J / PubMed:12941689 / PubMed Central
MethodsEM (single particle)
Resolution11.0 Å
Structure data

EMDB-1059:
Electron cryomicroscopy structure of N-ethyl maleimide sensitive factor at 11 A resolution.
Method: EM (single particle) / Resolution: 11.0 Å

Source
  • Cricetulus griseus (Chinese hamster)
  • Bos taurus (cattle)
  • unidentified (others)

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