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-Structure paper
| タイトル | Complete atomic model of the bacterial flagellar filament by electron cryomicroscopy. |
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| ジャーナル・号・ページ | Nature, Vol. 424, Issue 6949, Page 643-650, Year 2003 |
| 掲載日 | 2003年8月7日 |
著者 | Koji Yonekura / Saori Maki-Yonekura / Keiichi Namba / ![]() |
| PubMed 要旨 | The bacterial flagellar filament is a helical propeller for bacterial locomotion. It is a helical assembly of a single protein, flagellin, and its tubular structure is formed by 11 protofilaments in ...The bacterial flagellar filament is a helical propeller for bacterial locomotion. It is a helical assembly of a single protein, flagellin, and its tubular structure is formed by 11 protofilaments in two distinct conformations, L- and R-type, for supercoiling. The X-ray crystal structure of a flagellin fragment lacking about 100 terminal residues revealed the protofilament structure, but the full filament structure is still essential for understanding the mechanism of supercoiling and polymerization. Here we report a complete atomic model of the R-type filament by electron cryomicroscopy. A density map obtained from image data up to 4 A resolution shows the feature of alpha-helical backbone and some large side chains. The atomic model built on the map reveals intricate molecular packing and an alpha-helical coiled coil formed by the terminal chains in the inner core of the filament, with its intersubunit hydrophobic interactions having an important role in stabilizing the filament. |
リンク | Nature / PubMed:12904785 |
| 手法 | EM (らせん対称) |
| 解像度 | 4 Å |
| 構造データ | ![]() PDB-1ucu: |
| 由来 |
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キーワード | STRUCTURAL PROTEIN / FLAGELLIN / FLAGELLAR FILAMENT / HELICAL RECONSTRUCTION |
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万見文献について



著者
リンク
salmonella typhimurium (サルモネラ菌)
キーワード