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TitleStructural Basis for Ligand Binding and Processivity in Cellobiohydrolase Cel6A from Humicola Insolens
Journal, issue, pagesStructure, Vol. 11, Page 855-, Year 2003
Publish dateFeb 6, 2003 (structure data deposition date)
AuthorsVarrot, A. / Frandsen, T.P. / Von Ossowski, I. / Boyer, V. / Driguez, H. / Schulein, M. / Davies, G.J.
External linksStructure / PubMed:12842048
MethodsX-ray diffraction
Resolution1.11 - 1.75 Å
Structure data

PDB-1oc5:
D405N mutant of the CELLOBIOHYDROLASE CEL6A FROM HUMICOLA INSOLENS in complex with methyl-cellobiosyl-4-deoxy-4-thio-beta-D-cellobioside
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-1oc6:
structure native of the D405N mutant of the CELLOBIOHYDROLASE CEL6A FROM HUMICOLA INSOLENS at 1.5 angstrom resolution
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-1oc7:
D405N mutant of the CELLOBIOHYDROLASE CEL6A FROM HUMICOLA INSOLENS in complex with methyl-tetrathio-alpha-d-cellopentoside at 1.1 angstrom resolution
Method: X-RAY DIFFRACTION / Resolution: 1.11 Å

PDB-1ocb:
Structure of the wild-type cellobiohydrolase Cel6A from Humicolas insolens in complex with a fluorescent substrate
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

PDB-1ocj:
Mutant D416A of the CELLOBIOHYDROLASE CEL6A FROM HUMICOLA INSOLENS in complex with a THIOPENTASACCHARIDE at 1.3 angstrom resolution
Method: X-RAY DIFFRACTION / Resolution: 1.3 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-CA:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-ACT:
ACETATE ION

ChemComp-DMF:
DIMETHYLFORMAMIDE

ChemComp-FLG:
FLUORESCEINYLTHIOUREIDO

ChemComp-ACY:
ACETIC ACID

Source
  • humicola insolens (fungus)
KeywordsHYDROLASE / CELLULOSE DEGRADATION / CELLOBIOHYDROLASE / CELLULASE / GLYCOSIDE HYDROLASE FAMILY 6 / PROCESSIVE MECHANISM / PROCESSIVE MECHANISM GLYCOSIDE HYDROLASE FAMILY 6

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