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Structure paper

TitleCrystal structure of Human beta-hexosaminidase B: Understanding the molecular basis of Sandhoff and Tay-Sachs disease
Journal, issue, pagesJ. Mol. Biol., Vol. 327, Page 1093-1109, Year 2003
Publish dateJan 16, 2003 (structure data deposition date)
AuthorsMark, B.L. / Mahuran, D.J. / Cherney, M.M. / Zhao, D. / Knapp, S. / James, M.N.G.
External linksJ. Mol. Biol. / PubMed:12662933
MethodsX-ray diffraction
Resolution2.2 - 2.5 Å
Structure data

PDB-1nou:
Native human lysosomal beta-hexosaminidase isoform B
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-1now:
Human lysosomal beta-hexosaminidase isoform B in complex with (2R,3R,4S,5R)-2-Acetamido-3,4-Dihydroxy-5-Hydroxymethyl-Piperidinium Chloride (GalNAc-isofagomine)
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-1np0:
Human lysosomal beta-hexosaminidase isoform B in complex with intermediate analogue NAG-thiazoline
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

ChemComp-IFG:
(2R,3R,4S,5R)-2-ACETAMIDO-3,4-DIHYDROXY-5-HYDROXYMETHYL-PIPERIDINE

ChemComp-NGT:
3AR,5R,6S,7R,7AR-5-HYDROXYMETHYL-2-METHYL-5,6,7,7A-TETRAHYDRO-3AH-PYRANO[3,2-D]THIAZOLE-6,7-DIOL

Source
  • homo sapiens (human)
KeywordsHYDROLASE / (beta/alpha)8-barrel / homodimer / family 20 glycosidase

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