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Structure paper

TitleOligosaccharide and Sucrose Complexes of Amylosucrase. STRUCTURAL IMPLICATIONS FOR THE POLYMERASE ACTIVITY
Journal, issue, pagesJ. BIOL. CHEM., Vol. 277, Page 47741-47747, Year 2002
Publish dateSep 27, 2002 (structure data deposition date)
AuthorsSkov, L.K. / Mirza, O. / Sprogoe, D. / Dar, I. / Remaud-Simeon, M. / Albenne, C. / Monsan, P. / Gajhede, M.
External linksJ. BIOL. CHEM. / PubMed:12364331
MethodsX-ray diffraction
Resolution2 - 2.1 Å
Structure data

PDB-1mvy:
Amylosucrase mutant E328Q co-crystallized with maltoheptaose.
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-1mw0:
Amylosucrase mutant E328Q co-crystallized with maltoheptaose then soaked with maltoheptaose.
Method: X-RAY DIFFRACTION / Resolution: 2.01 Å

PDB-1mw1:
Amylosucrase soaked with 14mM sucrose.
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-1mw2:
Amylosucrase soaked with 100mM sucrose
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-1mw3:
Amylosucrase soaked with 1M sucrose
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-HOH:
WATER

ChemComp-DTT:
2,3-DIHYDROXY-1,4-DITHIOBUTANE

Source
  • neisseria polysaccharea (bacteria)
KeywordsTRANSFERASE / (beta-alpha)8 barrel / protein-sugar complex

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