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TitleAcyl-intermediate Structures of the Extended-spectrum Class A beta -Lactamase, Toho-1, in Complex with Cefotaxime, Cephalothin, and Benzylpenicillin.
Journal, issue, pagesJ. Biol. Chem., Vol. 277, Page 46601-46608, Year 2002
Publish dateSep 4, 2002 (structure data deposition date)
AuthorsShimamura, T. / Ibuka, A. / Fushinobu, S. / Wakagi, T. / Ishiguro, M. / Ishii, Y. / Matsuzawa, H.
External linksJ. Biol. Chem. / PubMed:12221102
MethodsX-ray diffraction
Resolution1.8 - 2.1 Å
Structure data

PDB-1iyo:
Toho-1 beta-Lactamase In Complex With Cefotaxime
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-1iyp:
Toho-1 beta-Lactamase In Complex With Cephalothin
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-1iyq:
Toho-1 beta-Lactamase In Complex With Benzylpenicillin
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-CEF:
CEFOTAXIME, C3' cleaved, open, bound form / antibiotic*YM

ChemComp-HOH:
WATER

ChemComp-CEP:
CEPHALOTHIN GROUP

ChemComp-PNM:
OPEN FORM - PENICILLIN G / antibiotic*YM

Source
  • escherichia coli (E. coli)
KeywordsHYDROLASE / extended-spectrum / ESBL / BETA-LACTAMASE / ACYL-ENZYME / cefotaxime / complex / cephalothin / benzylpenicillin / penicillin G

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