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-Structure paper
タイトル | Structure of the 80S ribosome from Saccharomyces cerevisiae--tRNA-ribosome and subunit-subunit interactions. |
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ジャーナル・号・ページ | Cell, Vol. 107, Issue 3, Page 373-386, Year 2001 |
掲載日 | 2001年11月2日 |
著者 | C M Spahn / R Beckmann / N Eswar / P A Penczek / A Sali / G Blobel / J Frank / |
PubMed 要旨 | A cryo-EM reconstruction of the translating yeast 80S ribosome was analyzed. Computationally separated rRNA and protein densities were used for docking of appropriately modified rRNA models and ...A cryo-EM reconstruction of the translating yeast 80S ribosome was analyzed. Computationally separated rRNA and protein densities were used for docking of appropriately modified rRNA models and homology models of yeast ribosomal proteins. The core of the ribosome shows a remarkable degree of conservation. However, some significant differences in functionally important regions and dramatic changes in the periphery due to expansion segments and additional ribosomal proteins are evident. As in the bacterial ribosome, bridges between the subunits are mainly formed by RNA contacts. Four new bridges are present at the periphery. The position of the P site tRNA coincides precisely with its prokaryotic counterpart, with mainly rRNA contributing to its molecular environment. This analysis presents an exhaustive inventory of an eukaryotic ribosome at the molecular level. |
リンク | Cell / PubMed:11701127 |
手法 | EM (単粒子) |
解像度 | 15.4 Å |
構造データ | EMDB-1076: |
由来 |
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