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Titlealpha-Lactalbumin (LA) stimulates milk beta-1,4-galactosyltransferase I (beta 4Gal-T1) to transfer glucose from UDP-glucose to N-acetylglucosamine. Crystal structure of beta 4Gal-T1 x LA complex with UDP-Glc.
Journal, issue, pagesJ. Biol. Chem., Vol. 276, Page 37665-37671, Year 2001
Publish dateJan 10, 2003 (structure data deposition date)
AuthorsRamakrishnan, B. / Shah, P.S. / Qasba, P.K.
External linksJ. Biol. Chem. / PubMed:11485999
MethodsX-ray diffraction
Resolution2 - 2.32 Å
Structure data

PDB-1nmm:
beta-1,4-galactosyltransferase mutant Cys342Thr complex with alpha-lactalbumin and GlcNAc
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-1nwg:
BETA-1,4-GALACTOSYLTRANSFERASE COMPLEX WITH ALPHA-LACTALBUMIN AND N-BUTANOYL-GLUCOAMINE
Method: X-RAY DIFFRACTION / Resolution: 2.32 Å

PDB-1o23:
CRYSTAL STRUCTURE OF LACTOSE SYNTHASE IN THE PRESENCE OF UDP-GLUCOSE
Method: X-RAY DIFFRACTION / Resolution: 2.32 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

ChemComp-HOH:
WATER

ChemComp-BGN:
2-(butanoylamino)-2-deoxy-beta-D-glucopyranose

ChemComp-MES:
2-(N-MORPHOLINO)-ETHANESULFONIC ACID / pH buffer*YM

ChemComp-MN:
Unknown entry

ChemComp-UPG:
URIDINE-5'-DIPHOSPHATE-GLUCOSE

ChemComp-UDP:
URIDINE-5'-DIPHOSPHATE / UDP*YM

Source
  • mus musculus (house mouse)
  • bos taurus (domestic cattle)
KeywordsTRANSFERASE ACTIVATOR/TRANSFERASE / beta1 / 4-galactosyltransferase / Cys342Thr mutation / alpha-lactalbumin complex / TRANSFERASE ACTIVATOR-TRANSFERASE COMPLEX / alpha-lactalbumin; beta1 / 4-galactosyltransferase; N-BUTANOYL-GLUCOAMINE / ALPHA-LACTALBUMIN; BETA / 1 / 4-GALACTOSYLTRANSFERASE; UDP-GLUCOSE

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