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Structure paper

TitleX-Ray Snapshots of Serine Protease Catalysis Reveal a Tetrahedral Intermediate
Journal, issue, pagesNat. Struct. Biol., Vol. 8, Page 689-, Year 2001
Publish dateApr 10, 2001 (structure data deposition date)
AuthorsWilmouth, R.C. / Edman, K. / Neutze, R. / Wright, P.A. / Clifton, I.J. / Schneider, T.R. / Schofield, C.J. / Hajdu, J.
External linksNat. Struct. Biol. / PubMed:11473259
MethodsX-ray diffraction
Resolution1.4 - 2.05 Å
Structure data

PDB-1hax:
Snapshots of serine protease catalysis: (A) acyl-enzyme intermediate between porcine pancreatic elastase and human beta-casomorphin-7 at pH 5
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-1hay:
Snapshots of serine protease catalysis: (B) acyl-enzyme intermediate between porcine pancreatic elastase and human beta-casomorphin-7 jumped to pH 10 for 10 seconds
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-1haz:
Snapshots of serine protease catalysis: (C) acyl-enzyme intermediate between porcine pancreatic elastase and human beta-casomorphin-7 jumped to pH 9 for 1 minute
Method: X-RAY DIFFRACTION / Resolution: 1.4 Å

PDB-1hb0:
Snapshots of serine protease catalysis: (D) acyl-enzyme intermediate between porcine pancreatic elastase and human beta-casomorphin-7 jumped to pH 10 for 2 minutes
Method: X-RAY DIFFRACTION / Resolution: 2.05 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

Source
  • sus scrofa (pig)
  • homo sapiens (human)
KeywordsHYDROLASE / SERINE PROTEINASE / HYDROLASE (SERINE PROTEINASE) / SERINE PROTEINASE/HYDROLASE SUBSTRATE / HYDROLASE-HYDROLASE SUBSTRATE COMPLEX / SERINE PROTEINASE-HYDROLASE SUBSTRATE COMPLEX

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