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TitlePeudoreversion of the Catalytic Activity of Y14F by the Additional Substitution(S) of Tyrosine with Phenylalanine in the Hydrogen Bond Network of Delta 5-3-Ketosteroid Isomerase from Pseudomonas Putida Biotype B
Journal, issue, pagesBiochemistry, Vol. 40, Page 6828-, Year 2001
Publish dateOct 10, 2000 (structure data deposition date)
AuthorsChoi, G. / Ha, N.-C. / Kim, M.-S. / Hong, B.-H. / Kim, D.-H. / Oh, B.-H. / Choi, K.Y.
External linksBiochemistry / PubMed:11389596
MethodsX-ray diffraction
Resolution1.8 - 2 Å
Structure data

PDB-1e97:
Crystal structure of ketosteroid isomerase from Pseudomonas putida ; triple mutant y16f/y32f/y57f
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-1ea2:
Pseudoreversion of the Catalytic Activity of Y14F by the Additional Tyrosine-to-Phenylalanine Substitution(s) in the Hydrogen Bond Network of Delta-5-3-Ketosteroid Isomerase from Pheudomonas putida Biotype B
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

Chemicals

ChemComp-HOH:
WATER

Source
  • pseudomonas putida (bacteria)
KeywordsISOMERASE / KETOSTEROID ISOMERASE / LBHB

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